Agilent small rna kit manual

Reagent Kit Guides The Reagent Kit Guides give you information on how to perform specific assays, including sample and chip preparation. Publication Number Title G Agilent Bioanalyzer Installation and Safety Manual G Agilent Bioanalyzer Maintenance and Troubleshooting Guide Publication Number Title. Single-Guide RNA (CRISPR) QC on // Fragment Analyzer using HS RNA Kit (DNF- ) and Small RNA Kit (DNF) Comparison of HS Small Fragment Kit (DNF‑) and HS NGS Fragment Kit (DNF‑) on the Agilent Fragment Analyzer. QC of IVT RNA on Fragment Analyzer using HS RNA Kit (DNF) and RNA Kit (DNF‑). 10 D-PLEX SMALL RNA-SE KIT MANUAL Remarks Before Starting General recommendations • Read the complete manual before first time use. • The D-Plex Small RNA-seq protocol is essentially the same for both UDI and SI library constructions. However, the RT Primer H, RT Primer M and Small Template Switching Oligo are specific to.

Data Analysis: RNA Ladders RNA Ladder is used for sizing and quantitation: 6 ladder peaks + Lower Marker Ladder concentration: RNA Nano: ng/µl Small RNA ladder: pg/µl RNA Pico: pg/µl Chip Summary Standard Curve: Lower marker Rev. 1 [Janu] For Research Use Only. Not for use in diagnostic procedures. For kit components of other NEBNext Small RNA Library Prep Sets for Illumina, check the appropriate pages in this manual. The NEBNext Multiplex Small RNA Prep Kit for Illumina (Index Primers ) Includes The volumes provided are sufficient for preparation of up to 96 reactions (NEB #ES). All reagents should be stored at °C. Alien Reference RNA QRT-PCR Detection Kit 1 Alien Reference RNA QRT-PCR Detection Kit MATERIALS PROVIDED Catalog #, Quantity Materials Provided Concentration Catalog # Catalog # Alien RNA Transcript 3 × copies/μl in mM EDTA/RNase-free H 2O 20 μl 20 μl Alien Probe Forward Primer μM μla μla.

Agilent DNF Small RNA Kit Quick Guide for Fragment Analyzer Systems. This Quick Guide is intended for use with the Agilent , , and Fragment Analyzer systems only. The Small RNA kit ( Samples) (Part # DNF) is designed for the sizing and quantification of Small RNA samples, and determination of microRNA region content. Agilent RNA kits contain chips and reagents designed for analysis of RNA fragments. Each RNA chip contains an interconnected set of microchannels that is used for separation of nucleic acid fragments based on their size as they are driven through it electrophoretically. Agilent RNA kits are designed for use with the Agilent Bioanalyzer instrument only. Manual Part Number G Rev. C Edition 12/ Printed in Germany Agilent Technologies Hewlett-Packard-Strasse 8 Waldbronn 1 Agilent RNA Pico Kit 4.